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Simultaneous determination of 35 constituents and elucidation of effective const

时间:2021-02-04   浏览次数:  分享到:

期刊名:Chemosphere

网站:https://doi.org/10.1016/j.chemosphere.2021.130444
发表日期:Volume 278, September 2021, 130444
Abstract

Xiaoer-Feire-Kechuan (XFK) is an 11-component Chinese medicine formula to treat cough and pulmonary inflammatory. The complicated composition rendered its chemical analysis and effective-component elucidation. In this study, we combined quantitative analysis and bioactivity test to reveal the anti-inflammatory constituents of XFK. Firstly, UPLC-DAD and UHPLC/Q-Orbitrap-MS methods were established and validated to quantify 35 compounds (covering 9 out of 11 component herbs) in different XFK formulations. Parallel Reaction Monitoring mode built-in Q-Orbitrap-MS was used to improve the sensitivity and selectively. Then, anti-inflammatory activities of the 35 compounds were analyzed using in vitroCOX-2 inhibition assay. Finally, major compounds mangiferin (6), forsythosides H, I, A (8-10), and baicalin (15) (total contents varied from 38.01 to 148.12 mg/dose in different formulations) with significant activities (inhibitory rate ≥ 80%) were proposed as the anti-inflammatory constituents of XFK. The present study provided an effective strategy to discover effective constituents of multi-herb formulas.

1, 2, 4, 6, 11, 12, 14, 19, 24,27, 28, 31-33, and 35 were purchased from Chengdu DeSiTe Biological Technology
Co., Ltd. (Chengdu, China);

 


 

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